3M™ Walnut Protein ELISA Kit, E96WAL, 96 wells/kit

The 3M™ Walnut Protein ELISA Kit is intended for the detection of walnut proteins in clean-in-place (CIP) final rinse water, environmental swab samples, food ingredients and processed food products.

The 3M Walnut Protein ELISA Kit utilizes a sandwich ELISA. The walnut proteins present in the sample react with the anti-walnut antibody, which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-walnut antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound walnut protein. Following a second washing step, the enzyme bound to the immunosorbent is detected by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The color development from this enzymatic reaction varies directly with the concentration of walnut protein in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of walnut protein in the test sample. The quantity of walnut protein in the test sample can be extrapolated from the standard curve, constructed from standards of known concentration, and adjusted to consider the sample dilution.

• Designed to detect both processed and unprocessed target allergen proteins.
• Test can be used for environmental swabs, CIP rinse water and food samples.
• A universal extraction protocol simplifies testing for multiple analytes.
• 3M™ Environmental Collection Swabs sold separately.